TE Buffer
组成浓度：10mM Tris-HCl
1mM EDTA PH=8.0
配制量：500ml
配制方法：量取下列溶液于500ml烧杯中

1M Tris-HCl Buffer PH=8.0 5ml
0.5M EDTA PH=8.0 1ml
向烧杯中加入约400mldd H2O均匀混合；将溶液定容到500ml后，高温高压灭菌；室温保存.

TE buffer
10 mM Tris-Cl, pH 7.5
1 mM EDTA
Make from 1M stock of Tris-Cl (pH 7.5) and 500 mM stock of EDTA (pH 8.0).
10ml 1M Tris-Cl pH 7.5 per L
2ml 500mM EDTA pH 8.0
1M Tris (crystallized free base)
Tris(hydroxymethyl) aminomethane
FW 121.4 g/mol
60.57 g in 0.5L mq water
pH to 7.5 using HCl
0.5M EDTA
Diaminoethane tetraacetic acid
FW 372.2 g/mol
18.6 g in 100ml mq water
pH to 8.0 using NaOH
• EDTA will not be soluble until pH reaches 8.0
• Use vigorous stirring, moderate heat (if desired) and time