For 1 litre of 1x phosphate-buffered saline (1x PBS buffer) use: 8.00 g of NaCl 0.20 g of KCl 1.44 g of Na2HPO4 0.24 g of KH2PO4 Dissolve in 800 ml of distilled H2O Adjust the pH to 7.4 with HCl or NaOH Add distilled ...
For 1 litre of 1x phosphate-buffered saline (1x PBS buffer) use: 8.00 g of NaCl 0.20 g of KCl 1.44 g of Na2HPO4 0.24 g of KH2PO4 Dissolve in 800 ml of distilled H2O Adjust the pH to 7.4 with HCl or NaOH Add distilled ...
引物在碱性条件下保存时间较长,双脱水偏酸性,DNA容易降解,TE Buffer pH在7.5-8左右,弱碱性,所以我们建议用1×TE Buffer 溶解引物,并保存于-20度。
TE Buffer组成浓度:10mM Tris-HCl 1mM EDTA PH=8.0配制量:500ml配制方法:量取下列溶液于500ml烧杯中 1M Tris-HCl Buffer PH=8.0 5ml 0.5M EDTA PH=8.0 1ml 向烧杯中加入约400mldd H2O均匀混合;将溶液定容到500ml后,高温高...
50X TAE Buffer Preparation (100mL) 试剂 终浓度 用量 备注 Tris 2 mol/L 24.2 g MW:121.14 Acetic Acid 1 mol/L 5.71 mL 17.4 mol/L EDTA 50 mmol/L 10 mL 0.5 M PH 8.0 加dH20至100 mL PS: 1. 此溶液可以用单蒸水...
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